Biolegend, 304906, PE anti-human CD62P (P-Selectin) Antibody, 100tests

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).
Application References(PubMed link indicates BioLegend citation): Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block) Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Yen YT, et al. 2006. J. Virol. 80:2684. Sato Y, et al. 2005. Blood 106:428. (IHC)
Product Citations: Buijsers B, et al. 2022. Front Immunol. 12:759570. PubMed Garcia-Larragoiti N, et al. 2021. J Thromb Haemost. 19:2572. PubMed Li L, et al. 2022. FEBS J. 289:2877. PubMed Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed Li L, et al. 2023. Br J Pharmacol. 180:287. PubMed Cano-Mendez A, et al. 2022. Int J Mol Sci. 23: . PubMed Machata S, et al. 2021. Front Immunol. 11:565869. PubMed Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed Artemenko E, et al. 2016. Biochem J. 473: 435 - 448. PubMed Best MG et al. 2017. Cancer cell. 32(2):238-252 . PubMed Sung PS, et al. 2019. Nat Commun. 10:2402. PubMed Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed Petersen-Uribe Á, et al. 2021. Int J Mol Sci. 22:. PubMed Zhang S, et al. 2012. PLoS One. 7:e40641. PubMed Bennett JA, et al. 2020. Nat Commun. 2.874305556. PubMed Lee S, et al. 2021. Nature. 599:283. PubMed Crooks M, et al. 2014. PLoS One. 9:111347. PubMed Welsh J, et al. 2011. Br J Haematol. 154:122. PubMed Marsden AJ, et al. 2021. ACS Pharmacol Transl Sci. 4:1567. PubMed Deng W, et al. 2016. Nat Commun. 7: 12863. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Karampini E, et al. 2019. Haematologica. 104:2091. PubMed
RRID: AB_314477 (BioLegend Cat. No. 304905) AB_314478 (BioLegend Cat. No. 304906)
Structure: Selectin, type I glycoprotein, 140 kD
Distribution: Activated platelets, megakaryocytes, endothelial cells
Function: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type: Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025. 2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.
Gene ID: 6403
UniProt: View information about CD62P on UniProt.org
Clone: AK4
Regulatory Status: RUO
Workshop: VI P-44
Other Names: GMP-140, PADGEM
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.