Biolegend, 305023, PerCP/Cyanine5.5 anti-human CD64 Antibody, 25tests

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12.
Application References(PubMed link indicates BioLegend citation): McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Holl V, et al. 2004. J. Immunol. 173:6274. Hober D, et al. 2002. J. Gen. Virol. 83:2169. Cho HJ, et al. 2007. Physiol Genomics 149:60. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed Bruhns P, et al. 2008. Blood 113:3716. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Carter DL, et al. 1999. Cytometry 37:41. (FC) Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC)
Product Citations: Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Ghiboub M, et al. 2022. BMC Biol. 20:182. PubMed Llewellyn D, et al. 2015. Sci Rep. 5: 14081. PubMed Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
RRID: AB_2561585 (BioLegend Cat. No. 305023) AB_2561586 (BioLegend Cat. No. 305024)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Hulett M, et al. 1994. Adv. Immunol. 57:1. 2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID: 2209
UniProt: View information about CD64 on UniProt.org
Clone: 10.1
Regulatory Status: RUO
Workshop: VI MA36
Other Names: FcγRI, FcR I, FCGR1A
Isotype: Mouse IgG1, κ
Q: Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
A: Yes
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.