Biolegend, 305219, APC anti-human CD80 Antibody, 25tests

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC) Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB) Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Redka DS, et al. 2018. Mol Biol Cell. 29:53. PubMed Siepe DH, et al. 2022. Elife. 11:. PubMed Murugesan G, et al. 2022. Biomedicines. 10:. PubMed Xu C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:327. PubMed Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed Hashimoto M, et al. 2015. J Immunol. 95: 4341 - 4350. PubMed Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed Škrnjug I, et al. 2014. PLoS One. 9:110150. PubMed Jost M, et al. 2021. eLife. 10:00. PubMed Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed Séguier S, et al. 2013. PLoS One. 8:70937. PubMed Douguet L, et al. 2016. PLoS One. 11:e0166164. PubMed Sohrabi Y, et al. 2019. Front Immunol. 9:3155. PubMed Chaumonnot K, et al. 2021. Cell Death Dis. 12:114. PubMed Amo L, et al. 2022. Front Immunol. 13:835527. PubMed Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed Zhang Z, et al. 2022. Clin Transl Med. 12:e1072. PubMed Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Katerina Gkirtzimanaki et al. 2018. Cell reports. 25(4):921-933 . PubMed Brummelman J, et al. 2015. Infect Immun . 83:227. PubMed Mertens C, et al. 2018. Oncoimmunology. 7:e1408751. PubMed
RRID: AB_2291403 (BioLegend Cat. No. 305219) AB_2076147 (BioLegend Cat. No. 305220)
Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 941
UniProt: View information about CD80 on UniProt.org
Clone: 2D10
Regulatory Status: RUO
Workshop: VI CD80.1
Other Names: B7-1, B7, BB1
Isotype: Mouse IgG1, κ