Biolegend, 305503, FITC anti-human CD94 Antibody, 25tests

CD94 is a 43 kD type II transmembrane glycoprotein also known as KP43. CD94 belongs to the C-type lectin superfamily and is present as a covalently linked heterodimer with NKG2 on the cell surface. CD94 is expressed by NK cells, a subset of γδ T cells, and NKT cells. The CD94/NKG2A complex serves as an inhibitory receptor specific for HLA-class I molecules.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NK cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5, immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7.
Application References(PubMed link indicates BioLegend citation): Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54. Phillip J, et al. 1996. Immunity 5:163. Lazetic S, et al. 1996. J. Immunol. 157:4741. Lanier LL, et al. 1998. Immunity 8:693. Wooden SL, et al. 2005. J. Immunol. 175:1383. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Hu X, et al. 2023. Nat Biotechnol. . PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed Wu J, et al. 2021. Front Immunol. 12:601611. PubMed Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed Mallis P, et al. 2020. World J Stem Cells. 12:203. PubMed Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
RRID: AB_314534 (BioLegend Cat. No. 305504)
Structure: C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD
Distribution: NK cells , T subset
Function: Inhibits NK function
Cell Type: NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165. 2. Moretta A, et al. 1997. Immunol. Rev. 155:105.
Gene ID: 3824
UniProt: View information about CD94 on UniProt.org
Clone: DX22
Regulatory Status: RUO
Other Names: KP43
Isotype: Mouse IgG1, κ