Biolegend, 305702, Purified anti-human CD95 (Fas) Antibody, 100μg

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis, and has been shown to play a role in the maintenance of peripheral tolerance. The EOS9.1 antibody is useful for inducing apoptosis of Fas-positive cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis. The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305705 & 305706).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
Product Citations: Raja S, et al. 2008. Mol Cancer Ther. 1.827777778. PubMed
RRID: AB_314554 (BioLegend Cat. No. 305702)
Structure: TNFR superfamily, type I transmembrane protein, 45 kD
Distribution: T cells and B cells, monocytes, neutrophils, expression level upregulated on activated lymphocytes
Function: Mediates apoptosis
Ligand/Receptor: Fas ligand (CD178)
Cell Type: B cells, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Krammer P, et al. 1994. Immunol. Rev. 142:175. 2. Nagata S, et al. 1995. Science 267:1449.
Gene ID: 355
UniProt: View information about CD95 on UniProt.org
Clone: EOS9.1
Regulatory Status: RUO
Other Names: Fas, APO-1, TNFRSF6
Isotype: Mouse IgM, κ