Biolegend, 306009, PE/Cyanine7 anti-human CD123 Antibody, 25tests

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The CD123 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
Application References(PubMed link indicates BioLegend citation): Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations: Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed Han L, et al. 2012. PLoS One. 4:e7989. PubMed Wieland S, et al. 2014. J Virol. 88:752.. PubMed Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed Royle C, et al. 2014. J Immunol. 193:3538. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Schwartz JA, et al. 2018. J Virol. 92:19. PubMed Kanakoudi-Tsakalidou F, et al. 2014. Lupus. 23:305. PubMed Greer A, et al. 2014. PLoS One. 9:99084. PubMed Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed Tillack K, et al. 2012. J Immunol. 188:3150. PubMed Hasan S, et al. 2017. Am J Physiol Cell Physiol. 312:C286. PubMed Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed Bziat V, et al. 2021. Cell. . PubMed Nilsson JS, et al. 2019. Oncol Lett. 2.484027778. PubMed Lu J, et al. 2021. Front Immunol. 12:732612. PubMed Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed Louka E, et al. 2021. Journal of Experimental Medicine. 218(2):. PubMed Rodriguez-Meira A, et al. 2020. STAR Protoc. 1:100125. PubMed Lv L, et al. 2019. Exp Ther Med. 17:1360. PubMed Chen YL, et al. 2020. J Exp Med. 217:. PubMed Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed Kivisäkk P, et al. 2014. PLoS One. 9:103716. PubMed Finotti G, et al. 2016. J Leukoc Biol. 99: 107 - 119. PubMed Rodrigues E, et al. 2020. Nat Commun. 3.99375. PubMed Suszczyk D, et al. 2022. Int J Mol Sci. 23:. PubMed Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed Bill M, et al. 2018. J Cell Mol Med. 22:2311. PubMed Ianniciello A, et al. 2021. Sci Transl Med. 13:eabd5016. PubMed Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
RRID: AB_493577 (BioLegend Cat. No. 306009) AB_493576 (BioLegend Cat. No. 306010)
Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563
UniProt: View information about CD123 on UniProt.org
Clone: 6H6
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ