Biolegend, 306012, APC anti-human CD123 Antibody, 100tests

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
Application References(PubMed link indicates BioLegend citation): Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations: Zhang X, et al. 2022. Commun Biol. 5:1332. PubMed Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed Kitai H, et al. 2022. BMC Biol. 20:248. PubMed Vijayakumar B, et al. 2022. Immunity. . PubMed González-Tajuelo R, et al. 2020. Front Immunol. 11:588212. PubMed Oliveira JAP, et al. 2021. J Leukoc Biol. 110:167. PubMed Ugawa M, et al. 2021. Elife. 10:. PubMed Thompson EA, et al. 2021. Cell Rep. 108863:34. PubMed Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed Lu J, et al. 2021. Front Immunol. 12:732612. PubMed Georgakis S, et al. 2021. JCI Insight. 6:. PubMed Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed Pierog PL, et al. 2018. J Immunol. 200:186. PubMed Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed Labidi-Galy S, et al. 2011. Cancer Res. 71:5423. PubMed Shevchenko JA, et al. 2020. Oncol Rep. 43:671. PubMed Conrad C, et al. 2018. Nat Commun. 9:25. PubMed
RRID: AB_439778 (BioLegend Cat. No. 306011) AB_439779 (BioLegend Cat. No. 306012)
Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563
UniProt: View information about CD123 on UniProt.org
Clone: 6H6
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ