Biolegend, 306013, FITC anti-human CD123 Antibody, 25tests

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
Application References(PubMed link indicates BioLegend citation): Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations: Hess NJ, et al. 2020. J Immunol. 205:272. PubMed Murayama G,et al. 2017. Arthritis Res Ther.. 10.1186/s13075-017-1441-7. PubMed Hagen SH, et al. 2020. Cell Rep. 33:108485. PubMed Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed Ishimori A, et al. 2016. Allergol Int. pii: S1323-8930(16)30105-8. PubMed Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Imai Y, et al. 2021. JID Innov. 1:100003. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Shi B, et al. 2012. PLoS One. 7:e44900. PubMed Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed Pritchard A, et al. 2014. PLoS One. 9:106501. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Hagen SH, et al. 2021. STAR Protocols. 2(3):100641. PubMed Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Matsuyama H, et al. 2019. Sci Rep. 9:13181. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID: AB_755989 (BioLegend Cat. No. 306013) AB_2124259 (BioLegend Cat. No. 306014)
Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563
UniProt: View information about CD123 on UniProt.org
Clone: 6H6
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ