Biolegend, 306016, PerCP/Cyanine5.5 anti-human CD123 Antibody, 100tests

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
Application References(PubMed link indicates BioLegend citation): Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations: Li M, et al. 2023. Front Immunol. 14:1087923. PubMed Ait Djebbara S, et al. 2023. Front Immunol. 14:1180900. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Ferrari B, et al. 2022. J Clin Invest. 132:. PubMed Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed Laing AG, et al. 2020. Nat Med. 26:1623. PubMed Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed Jankeel A, et al. 2020. J Virol. :94. PubMed Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed Manuel S, et al. 2013. AIDS Res Hum Retroviruses. 29:1273. PubMed Li M, et al. 2020. Nat Commun. 4051:11. PubMed Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed
RRID: AB_2124258 (BioLegend Cat. No. 306015) AB_2264693 (BioLegend Cat. No. 306016)
Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563
UniProt: View information about CD123 on UniProt.org
Clone: 6H6
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.