Biolegend, 306101, Purified anti-human CD131 Antibody, 25μg

CD131, also known as the IL-3R common β subunit (β C ), is a 95-120 kD type I transmembrane glycoprotein and belongs to the Ig superfamily. The common β subunit associates with the specific α subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response. The 1C1 antibody is a non-blocking antibody.
25μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3R β chain transfected COS cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1,2 and immunoprecipitation2. The 1C1 antibody does not block binding of IL-3 and is a non-neutralizing antibody.
Application References(PubMed link indicates BioLegend citation): Stomski F, et al. 1998. J. Biol. Chem. 273:1192. (WB) Stomski F, et al. 1999. Blood 94:1933. (IP WB) Mark A, et al. 2000. Mol. Cell 6:99.
RRID: AB_2229992 (BioLegend Cat. No. 306102)
Structure: Ig superfamily, type I transmembrane glycoprotein, associates with the α subunit of IL-3, IL-5, GM-CSF receptors, 95-120 kD
Distribution: Low levels on monocytes, granulocytes, eosinophils, basophils, hematopoietic progenitors, endothelial cells
Function: Signal transducing molecule of IL-3, IL-5, GM-CSF receptors
Ligand/Receptor: IL-3, IL-5, GM-CSF
Cell Type: Basophils, Endothelial cells, Eosinophils, Granulocytes, Hematopoietic stem and progenitors, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sun Q, et al. 1999. Blood 94:1943. 2. Woodcock J, et al. 1997. Blood 90:3005. 3. Lopez A, et al. 1991. J. Biol. Chem. 266:24741.
Gene ID: 1439
UniProt: View information about CD131 on UniProt.org
Clone: 1C1
Regulatory Status: RUO
Other Names: IL-3R common β chain, CDw131
Isotype: Mouse IgG1, κ