Biolegend, 307302, Purified anti-human CD262 (DR5, TRAIL-R2) Antibody, 100μg

DR5 is 55 kD member 10B of the TNF receptor superfamily (TNFRSF10B), also known as TRAIL-R2, TRICK2, KILLER, and CD262. It binds the cytotoxic ligand TRAIL and induces apoptosis. The DR5 receptor is broadly expressed on a variety of normal tissues and many tumors. DR5 expression has been reported to be upregulated in human cells by interferon-α, 2-methoxyestradiol, and paclitaxel, and downregulated by adenoviral E3 proteins.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of DR5-human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For successful immunofluorescent staining results, it may be important to maximize signal over background by using a high sensitivity, three-layer staining technique (e.g., biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Uno K, et al. 2003. Blood 101:3658. Sato K, et al. 2005. J. Immunol. 174:4025. Shi J, et al. 2008. Blood 111:1309.PubMed Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
Product Citations: Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed Winterberg D, et al. 2021. J Clin Med. 10:. PubMed Mitchell M, et al. 2017. Nat Commun. 10.1038/ncomms14179. PubMed Shi J, et al. 2008. Blood. 111:1309. PubMed
RRID: AB_314672 (BioLegend Cat. No. 307302)
Structure: TNFR superfamily, 55 kD
Distribution: Broadly expressed on normal and tumor cells
Function: Induces apoptosis
Ligand/Receptor: TRAIL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. MacFarlane M, et al. 1997. J. Biol. Chem. 272:25417. 2. Walczak H, et al. 1997. EMBO J. 16:5386. 3. Shigeno M, et al. 2003. Oncogene 22:1653. 4. LaVallee T, et al. 2003. Cancer Res. 63:468. 5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759
Gene ID: 8795
UniProt: View information about CD262 on UniProt.org
Clone: DJR2-2 (2-6)
Regulatory Status: RUO
Other Names: TRAIL-R2, KILLER, TRICK2, TNFRSF10B, Ly98, CD262
Isotype: Mouse IgG1, κ