Biolegend, 307401, Purified anti-human CD262 (DR5, TRAIL-R2) Antibody, 25μg

DR5 is 55 kD member 10B of the TNF receptor superfamily (TNFRSF10B), also known as TRAIL-R2, TRICK2, KILLER, and CD262. It binds the cytotoxic ligand TRAIL and induces apoptosis. The DR5 receptor is broadly expressed on a variety of normal tissues and many tumors. DR5 expression has been reported to be upregulated in human cells by interferon-α, 2-methoxyestradiol, and paclitaxel, and downregulated by adenoviral E3 proteins.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of DR5-human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Uno K, et al. 2003. Blood 101:3658. Sato K, et al. 2005. J. Immunol. 174:4025. Lundgvist A,et al.2006.Cancer Res.14:7317.PubMed
Product Citations: Bruhs A, et al. 2015. J Invest Dermatol. 435:135. PubMed Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed
RRID: AB_314674 (BioLegend Cat. No. 307402)
Structure: TNFR superfamily, 55 kD
Distribution: Broadly expressed on normal and tumor cells
Function: Induces apoptosis
Ligand/Receptor: TRAIL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. MacFarlane M, et al. 1997. J. Biol. Chem. 272:25417. 2. Walczak H, et al. 1997. EMBO J. 16:5386. 3. Shigeno M, et al. 2003. Oncogene 22:1653. 4. LaVallee T, et al. 2003. Cancer Res. 63:468. 5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759
Gene ID: 8795
UniProt: View information about CD262 on UniProt.org
Clone: DJR2-4 (7-8)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: TRAIL-R2, KILLER, TRICK2, TNFRSF10B, Ly98, CD262
Isotype: Mouse IgG1, κ