Biolegend, 307505, PE/Cyanine7 anti-human CD161 Antibody, 25tests

CD161 is a type II transmembrane glycoprotein, also known as NKR-P1A, that is expressed as a 40-44 kD homodimer. It is a member of the C-type lectin superfamily. CD161 is expressed on a majority of NK cells, NKT cells, and subsets of peripheral T cells and CD3+ thymocytes. It has been reported that Th17 cells are a subpopulation of CD4+CD161+CCR6+ cells. While the biological function of CD161 is not clear, it has been suggested to serve either as a stimulatory receptor or to inhibit NK cell-mediated cytotoxicity and cytokine production. LLT-1 (lectin-like transcript-1, also named as osteoclast inhibitory lectin or CLEC2D) is the ligand of CD161.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD161 transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2922542 (BioLegend Cat. No. 307505) AB_2922542 (BioLegend Cat. No. 307506)
Structure: Type II glycoprotein, 40-44 kD homodimer, C-type lectin superfamily
Distribution: NK cells, T subset, subset of CD3+ thymocytes
Ligand/Receptor: LLT-1 (Lectin-like transcript-1)
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules
Antigen References: Takahashi T, et al. 2006. J Immunol. 176:211-6. Cosmi L, et al. 2008. J Exp Med. 205:1903-16. Aldemir H, et al. 2005. J Immunol. 175:7791-5. Rosen DB, et al. 2008. J Immunol. 180:6508-17.
Gene ID: 3820
UniProt: View information about CD161 on UniProt.org
Clone: W18070C
Regulatory Status: RUO
Other Names: NKR-P1A
Isotype: Rat IgG2a, κ