Biolegend, 308302, Purified anti-human CD255 (TWEAK) Antibody, 100μg

TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: hTWEAK/2PK-3 transfectant cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by sandwich ELISA. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). ELISA Detection: The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture. Neutralization1,2: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307).
Application References(PubMed link indicates BioLegend citation): Nakayama, M., et al. 2000. J. Exp. Med. 192:1373. (Neut) Nakayama, M., et al. 2003. J. Immunol. 170:341. (Neut) Zou H, et al. 2011. Gynecol Oncol.123:123. PubMed.
Product Citations: Loi M, et al. 2019. Methods Mol Biol. 455:1880. PubMed Zou H, et al. 2011. Gynecol Oncol. 123:123. PubMed
RRID: AB_314706 (BioLegend Cat. No. 308302)
Structure: TNF superfamily; 17 kD (Mammalian)
Distribution: Normal tissue and tumor cells at low levels
Function: Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Bioactivity: Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Cell Sources: Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cell Targets: Macrophages, astrocytes, endothelial cells
Receptors: Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401. 3. Kaplan, M., et al. 2000. J. Immunol. 164:2897. 4. Saas, P., et al. 2000. Glia 32:102. 5. Wiley, S., et al. 2001. Immunity 15:837.
Regulation: Type II integral membrane protein readily cleaved from cell surface
Gene ID: 8742
UniProt: View information about CD255 on UniProt.org
Clone: CARL-1
Regulatory Status: RUO
Other Names: TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Isotype: Mouse IgG3, κ