Biolegend, 308503, PE anti-human IFN-γ R β chain Antibody, 25tests

IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Hamster
Immunogen: soluable extracellular domains of the human IFN-γ R β chain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308506).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB) Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB) Joseph TD, et al. 2001. J. Biol. Chem. 276:47136. Choi JC, et al. 2007. J. Immunol. 178:1598. Tjon AS, et al. 2014. J Immunol. 192:5625. PubMed
Product Citations: Martínez-Sabadell A, et al. 2022. Cell Rep. 41:111430. PubMed Tjon A, et al. 2014. J Immunol. 192:5625. PubMed Kulling PM, et al. 2018. PLoS One. 13:e0193429. PubMed Ferrando-Martinez S, et al. 2018. J Clin Invest. 128:2089. PubMed Dela Peña–Ponce MG, et al. 2017. Front Pediatr. 5:15. PubMed Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
RRID: AB_314718 (BioLegend Cat. No. 308504)
Structure: Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
Distribution: All cells, except erythrocytes, at low levels
Function: Transducing signals
Ligand/Receptor: IFN-γ
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305. 2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.
Gene ID: 3459
UniProt: View information about IFN-gamma R beta chain on UniProt.org
Clone: 2HUB-159
Regulatory Status: RUO
Workshop: VI C-111
Other Names: IFN-γRβ, IFNγAF-1
Isotype: Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.