Biolegend, 309415, Alexa Fluor® 647 anti-human CD275 (B7-H2, ICOSL) Antibody, 25tests

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human B7H2-mIg fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.
RRID: AB_2810465 (BioLegend Cat. No. 309415) AB_2810466 (BioLegend Cat. No. 309416)
Structure: Ig superfamily, B7 family, 40 kD
Distribution: B cells and dendritic cells
Function: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand/Receptor: ICOS
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Wang S, et al. 2002. J. Biol. Chem. 96:2808. Wong SC, et al. 2003. Blood 102:1831.
Gene ID: 23308
UniProt: View information about CD275 on UniProt.org
Clone: 2D3
Regulatory Status: RUO
Other Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Isotype: Mouse IgG2b, κ