Biolegend, 309802, Purified anti-human CD137 (4-1BB) Antibody, 100μg

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
100μg
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ectodomain of recombinant human 4-1BB fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP) Salih HR, et al. 2000. J. Immunol. 165:2903. (FA) Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA) Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations: Law JC, et al. 2021. J Immunol. 206:37. PubMed Lu X, et al. 2021. J Exp Med. 218: . PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed Sun R, et al. 2022. Cells. 11:. PubMed Heesters BA, et al. 2021. J Exp Med. 218:. PubMed Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed Tang JS, et al. 2020. Food Funct. 11:5782. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed Ichikawa J, et al. 2020. Clin Cancer Res. 26:3384. PubMed Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID: AB_314781 (BioLegend Cat. No. 309802)
Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand
Cell Type: T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gruss H, et al. 1995. Blood 85:3378. 2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355. 3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219. 4. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: 4B4-1
Regulatory Status: RUO
Workshop: VI C-7
Other Names: 4-1BB, ILA, CD137, TNFRSF9
Isotype: Mouse IgG1, κ