Biolegend, 309819, Brilliant Violet 421™ anti-human CD137 (4-1BB) Antibody, 25tests

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ectodomain of recombinant human 4-1BB fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP) Salih HR, et al. 2000. J. Immunol. 165:2903. (FA) Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA) Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations: Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed Sulejmani O, et al. 2021. Cancers (Basel). 13:. PubMed Balachandran H, et al. 2022. Cell Rep. 38:110345. PubMed Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Li Y, et al. 2022. J Med Virol. 94:3998. PubMed Koutsakos M, et al. 2022. Immunity. 55:1316. PubMed Babl N, et al. 2023. Front Oncol. 13:1107484. PubMed Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed Harris MJ, et al. 2021. Mol Syst Biol. 17:e10091. PubMed Hu W, et al. 2021. Vaccines (Basel). 9:. PubMed Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed Andersch L, et al. 2019. BMC Cancer. 19:895. PubMed Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed Vitanza NA, et al. 2021. Nature Medicine. :. PubMed Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed Sekine T, et al. 2020. Cell. 183:158. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed Genoyer E, et al. 2019. J Virol. 93:e01579-18. PubMed
RRID: AB_10895902 (BioLegend Cat. No. 309819) AB_2563830 (BioLegend Cat. No. 309820)
Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand
Cell Type: T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gruss H, et al. 1995. Blood 85:3378. 2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355. 3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219. 4. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: 4B4-1
Regulatory Status: RUO
Workshop: VI C-7
Other Names: 4-1BB, ILA, CD137, TNFRSF9
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.