Biolegend, 309826, PE/Dazzle™ 594 anti-human CD137 (4-1BB) Antibody, 100tests

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ectodomain of recombinant human 4-1BB fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP) Salih HR, et al. 2000. J. Immunol. 165:2903. (FA) Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA) Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations: Sannier G, et al. 2023. Cell Rep Med. 4:100955. PubMed Nayrac M, et al. 2022. Cell Rep. 39:111013. PubMed Nicolas A, et al. 2023. iScience. 26:105904. PubMed de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed Stephenson E, et al. 2021. Nat Med. 27:904. PubMed Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed Yang W, et al. 2019. Nat Med. 25:767. PubMed Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Tauzin A, et al. 2021. Cell Host Microbe. . PubMed Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
RRID: AB_2566259 (BioLegend Cat. No. 309825) AB_2566260 (BioLegend Cat. No. 309826)
Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand
Cell Type: T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Gruss H, et al. 1995. Blood 85:3378. 2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355. 3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219. 4. Schwarz H, et al. 1996. Blood 87:2839.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: 4B4-1
Regulatory Status: RUO
Workshop: VI C-7
Other Names: 4-1BB, ILA, CD137, TNFRSF9
Isotype: Mouse IgG1, κ