Biolegend, 310009, Purified anti-human CD16 Antibody, 100μg

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD16 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Activ - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W18182B can completely block the binding of clone B73.1 on target cells. Clone W18182B does not affect the binding of clones 3G8 or W18182A on target cells. Clone W18182B is able to activate NK cells and induce degranulation.
RRID: AB_3720492 (BioLegend Cat. No. 310009)
Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: Expressed on natural killer cells, activated monocytes, macrophages, neutrophils and a subset of T cells
Function: Low affinity IgG Fc receptor
Ligand/Receptor: IgG Fc (Fcγ)
Cell Type: Granulocytes, Neutrophils, NK cells
Biology Area: Adaptive Immunity, Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711. Ravetch JV and Bolland S. 2001. Annu Rev Immunol. 19:275.
Gene ID: 22142215
UniProt: View information about CD16 on UniProt.org
Clone: W18182B
Regulatory Status: RUO
Other Names: FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype: Rat IgG1, κ