Biolegend, 310403, PE anti-human CD129 (IL-9 R) Antibody, 25tests

CD129 is known as the 57 kD IL-9 receptor. It is a member of the hematopoietin receptor superfamily. Although the α-chain of the receptor binds IL-9 with high affinity, interaction with the γ-chain (CD132) of the IL-2 receptor is required for signaling. The IL-9 receptor is expressed at low levels on eosinophils, mast cells, macrophages, B lymphocytes, T lymphocytes, and erythroid progenitors. IL-9 receptor binding initiates STAT activation required for the proliferative and anti-apoptotic effects of this cytokine. In humans, signals from the IL-9 receptor appear to be critical for intrathymic T cell development. IL-9 binding has been shown to increase IL-5 receptor expression and promote survival in human eosinophils.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: inhibition the binding of IL-9 to the high affinity a-chain of the human IL-9 receptor1,2, and ELISA for detection of soluble IL-9R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 310404) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310408).
Application References(PubMed link indicates BioLegend citation): De Smedt M, et al. 2000. J. Immunol. 164:1761. Pilette C, et al. 2002. J. Immunol. 168:4103. Demoulin JB, et al.1996. Mol. Cell. Biol. 16 (9):4710.
Product Citations: Das S, et al. 2021. J Immunol. 206:2740. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Xiao M, et al. 2017. Blood. 129:3196. PubMed
RRID: AB_314816 (BioLegend Cat. No. 310403) AB_314817 (BioLegend Cat. No. 310404)
Structure: Cytokine receptor family, type I membrane protein or soluble, associate with common γ chain(CD132), 57 kD
Distribution: Eosinophils, mast, thymocytes, erythroid, myeloid progenitors, macrophages
Function: Activates STAT; critical for intrathymic T cell development
Ligand/Receptor: IL-9
Cell Type: Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Demoulin JB, et al.1996. Mol. Cell. Biol. 16:4710.
Gene ID: 3581
UniProt: View information about CD129 on UniProt.org
Clone: AH9R7
Regulatory Status: RUO
Other Names: CD129, IL-9R
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.