Biolegend, 310838, Brilliant Violet 711™ anti-human CD154 (CD40L) Antibody, 100tests

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF) Rushworth SA, et al. 2002. Transplantation 73:635. (Block) Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF) Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block) Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed Kuchen S, et al. 2007. J. Immunol. 179:5886. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Newton HS, et al. 2023. Front Allergy. 1126012:4. PubMed Newton HS, et al. 2024. Methods Mol Biol. 245:2789. PubMed Newton HS, et al. 2024. Methods Mol Biol. 269:2789. PubMed Apostolidis SA, et al. 2021. Nat Med. 27:1990. PubMed Painter MM, et al. 2021. Immunity. 54:2133. PubMed Zhang P, et al. 2021. Nat Med. 22:. PubMed Lederer K, et al. 2022. Cell. . PubMed Vanoni G, et al. 2021. eLife. 10:00. PubMed Zelba H, et al. 2021. J Immunol. 206:580. PubMed
RRID: AB_2563844 (BioLegend Cat. No. 310837) AB_2563845 (BioLegend Cat. No. 310838)
Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227. 2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275. 3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959
UniProt: View information about CD154 on UniProt.org
Clone: 24-31
Regulatory Status: RUO
Other Names: CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype: Mouse IgG1, κ