Biolegend, 311418, Pacific Blue™ anti-human HLA-A,B,C Antibody, 100μg

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells. They play an important role in cell-mediated immune responses and tumor surveillance.
100μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1..0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Darrow TL, et al. 1989. J. Immunol. 142:3329. Stern P, et al. 1987. J. Immunol. 138:1088. Tran TM, et al. 2001. Immunogenetics 53:440. Barbatis C, et al. 1981. Gut 22:985. Ayyoub M, et al. 2004. Cancer Immunity 4:7. DeFelice M, et al. 1990. Cell. Immunol. 126:420. Fayen J, et al. 1998. Int. Immunol. 10:1347. Turco MC, et al. 1988. J. Immunol. 141:2275. Geppert TD, et al. 1989. J. Immunol. 142:3763. Wooden SL, et al. 2005. J. Immunol. 175:1383. Nagano M, et al. 2007. Blood 110:151. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope) Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations: Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed Bettencourt P, et al. 2020. NPJ Vaccines. 5:2. PubMed Yaqubi M, et al. 2022. Glia. 70:1938. PubMed Io S, et al. 2021. STAR Protoc. 2:100921. PubMed Martrus G, et al. 2016. J Virol. 90: 9018 - 9028. PubMed Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed Bettencourt P, et al. 2020. NPJ Vaccines. 5:02. PubMed Forbester JL, et al. 2020. J Virol. 94:. PubMed Kunitomi A, et al. 2022. Cell Rep Methods. 2:100317. PubMed Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed Delitto D, et al. 2015. Cancer Immunol Immunother. 64: 1553-63. PubMed Io S, et al. 2021. Cell Stem Cell. 28(6):1023-1039.e13. PubMed Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed Paulikat AD, et al. 2022. J Innate Immun. 14:569. PubMed Parker R, et al. 2021. Cell Reports. 35(8):109179. PubMed van Stigt Thans T, et al. 2020. Journal of Virology. 93(20):e00719-19.. PubMed Manuel S, et al. 2013. AIDS Res Hum Retroviruses. 29:1273. PubMed de Witte S, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0590-6. PubMed Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed Clutton G, et al. 2016. Sci Rep. 6:30749. PubMed
RRID: AB_493668 (BioLegend Cat. No. 311417) AB_493669 (BioLegend Cat. No. 311418)
Structure: Ig superfamily
Distribution: All nucleated cells
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 3105
UniProt: View information about HLA-A on UniProt.org
Clone: W6/32
Regulatory Status: RUO
Other Names: Major Histocompatibility Class I, MHC class I
Isotype: Mouse IgG2a, κ