Biolegend, 311504, PE anti-human CD137L (4-1BB Ligand) Antibody, 100tests

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed
Product Citations: Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed Neo SY, et al. 2019. J Clin Invest. 130:1185. PubMed Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed Vidard L, et al. 2019. J Immunol. 203:676. PubMed
RRID: AB_314882 (BioLegend Cat. No. 311503) AB_314883 (BioLegend Cat. No. 311504)
Structure: TNF superfamily, 97 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T cell proliferation, cytokine production
Ligand/Receptor: 4-1BB (CDw137)
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Akiba H, et al. 2000. J. Exp. Med. 191:375. 2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488. 3. DeBenedette MA, et al. 1997. J. Immunol. 158:551. 4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.
Gene ID: 8744
UniProt: View information about CD137L on UniProt.org
Clone: 5F4
Regulatory Status: RUO
Other Names: 4-1BBL, CD137L, TNFSF9, 41BBL
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.