Biolegend, 312711, Alexa Fluor® 700 anti-human CD158e1 (KIR3DL1, NKB1) Antibody, 25μg

CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NK cell clone VL186-1.6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312710).
Application References(PubMed link indicates BioLegend citation): Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP) Gumperz J, et al. 1996. J. Exp. Med. 183:1817. Gardiner CM, et al. 2001. J. Immunol. 166:2992. Bakker ABH, et al. 1998. J. Immunol. 160:5239. Goodier M, et al. 2000. J. Immunol. 165:139. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC) Yawata M, et al. 2002. Immunogenetics 54:543. Valiante NM, et al. 1997. Immunity 7:739. Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
Product Citations: Manser AR, et al. 2019. J Immunol. 203:2301. PubMed Sekine T, et al. 2016. Blood. 128: 297 - 312. PubMed Björkström N, et al. 2011. J Exp Med. 208:13:00. PubMed Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed Cosgrove C, et al. 2014. PLoS One. 9:105950. PubMed Fauriat C, et al. 2010. Blood. 115:1166. PubMed Miller J, et al. 2014. Biol Blood Marrow Transplant. 20:1252. PubMed Bziat V, et al. 2021. Cell. . PubMed Foley B, et al. 2012. Blood. 119:2665. PubMed Naluyima P, et al. 2019. J Immunol. 203:2210. PubMed Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID: AB_2234188 (BioLegend Cat. No. 312711) AB_2130824 (BioLegend Cat. No. 312712)
Structure: Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
Distribution: Subset of NK and T cells
Function: Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
Ligand/Receptor: HLA-B antigens such as the HLA-Bw4 allele
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Colonna M, et al. 1995. Science 268:405. 2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306. 3. Uhrburg M, et al. 1997. Immunity 7:753. 4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817. 5. Wagtmann N, et al. 1995. Immunity 3:801.
Gene ID: 3811
UniProt: View information about CD158e1 on UniProt.org
Clone: DX9
Regulatory Status: RUO
Other Names: NKAT3, NKB1, KIR-NKB1, p70
Isotype: Mouse IgG1, κ