Biolegend, 313201, Purified anti-human CD117 (c-kit) Antibody, 25μg

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
25μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MOLM-1 megakaryocytic cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Broudy VC, et al. 1999. Blood 94:1979. (IF, IP) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Lowinus T, et al. 2019. Cell Commun Signal. 17:05. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed Vigneault P, et al. 2018. J Physiol. 596:2359. PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed Nagano M, et al. 2007. Blood . 110:151. PubMed Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed Guo A, et al. 2011. J Pharmacol Exp Ther. 338:421. PubMed Ardain A, et al. 2019. Nature. 570:528. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed
RRID: AB_314980 (BioLegend Cat. No. 313201) AB_314981 (BioLegend Cat. No. 313202)
Structure: Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution: Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
Function: Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor: c-Kit ligand
Modification: Multiple phosphorylation sites
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Giebel LB, et al. 1992. Oncogene 7:2207. 2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
Gene ID: 3815
UniProt: View information about CD117 on UniProt.org
Clone: 104D2
Regulatory Status: RUO
Other Names: Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype: Mouse IgG1, κ