Biolegend, 313206, APC anti-human CD117 (c-kit) Antibody, 100tests

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MOLM-1 megakaryocytic cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Broudy VC, et al. 1999. Blood 94:1979. (IF, IP) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed Gordon E, et al. 2016. Proc Natl Acad Sci U S A. 113: 8765 - 8770. PubMed Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed Lu B, et al. 2018. Cancer Cell. 34:970. PubMed Frazier T, et al. 2018. Adipocyte. 0.41875. PubMed Imai Y, et al. 2021. JID Innov. 1:100003. PubMed Mandl M, et al. 2014. PLoS One. 9:112140. PubMed Leibel SL, et al. 2022. iScience. 25:103797. PubMed Ruan S, et al. 2020. Front Pharmacol. 11:150. PubMed Sputova K, et al. 2013. Genome Integr. 29:4. PubMed Ran L, et al. 2018. Cancer Discov. 8:234. PubMed Luni C, et al. 2011. Biotechnol Prog. 27:1154. PubMed Raman S, et al. 2021. Cell Reports Medicine. 2(7):100344. PubMed Zou T, et al. 2019. Nat Commun. 10:1205. PubMed Shalabi SF, et al. 2021. Nat Aging. 1:838. PubMed He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed Engeroff P, et al. 2020. J Allergy Clin Immunol. 145:301. PubMed Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
RRID: AB_314984 (BioLegend Cat. No. 313205) AB_314985 (BioLegend Cat. No. 313206)
Structure: Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution: Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
Function: Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor: c-Kit ligand
Modification: Multiple phosphorylation sites
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Giebel LB, et al. 1992. Oncogene 7:2207. 2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
Gene ID: 3815
UniProt: View information about CD117 on UniProt.org
Clone: 104D2
Regulatory Status: RUO
Other Names: Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype: Mouse IgG1, κ