Biolegend, 313212, PE/Cyanine7 anti-human CD117 (c-kit) Antibody, 100tests

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MOLM-1 megakaryocytic cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Broudy VC, et al. 1999. Blood 94:1979. (IF, IP) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed Fang F, et al. 2020. Front Cell Dev Biol. 0.633333333. PubMed Thomas A, et al. 2021. Cancer Cell. 39(4):566-579.e7. PubMed Skovdahl HK, et al. 2021. Frontiers in Pharmacology. 12:679741. PubMed Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed Allan J, et al. 2017. Leuk Lymphoma. 10.1080/10428194.2017.1352089. PubMed Liu S, et al. 2021. Mol Ther. 29:1918. PubMed Kerr BA, et al. 2021. Experimental Cell Research. 399(2):112456. PubMed Liao Y, et al. 2018. Stem Cells Transl Med. 0.659722222. PubMed Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed Gall M, et al. 2015. Mol Cancer Ther. 14: 2595 - 2605. PubMed Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed Blomme EE, et al. 2021. Clin Transl Immunology. 10:e1287. PubMed Ogaki S, et al. 2015. Sci Rep. 5:17297. PubMed Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
RRID: AB_893228 (BioLegend Cat. No. 313211) AB_893222 (BioLegend Cat. No. 313212)
Structure: Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution: Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
Function: Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor: c-Kit ligand
Modification: Multiple phosphorylation sites
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Giebel LB, et al. 1992. Oncogene 7:2207. 2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
Gene ID: 3815
UniProt: View information about CD117 on UniProt.org
Clone: 104D2
Regulatory Status: RUO
Other Names: Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype: Mouse IgG1, κ