Biolegend, 313332, Alexa Fluor® 647 anti-human CD135 (Flt-3/Flk-2) Antibody, 100tests

CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML-BC. CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BV-173 pro-B cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
RRID: AB_3683326 (BioLegend Cat. No. 313331) AB_3683326 (BioLegend Cat. No. 313332)
Structure: Member of the immunoglobulin supergene family, type 3 tyrosine kinase receptor; 130-160 kD
Distribution: Expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. Also expressed on AML, ALL and CML-BC tumor cells
Function: Tyrosine kinase growth factor receptor involved in the growth and differentiation of primitive hematopoietic cells
Ligand/Receptor: FLT3 ligand
Cell Type: B cells, Hematopoietic stem and progenitors, Leukemia, Thymocytes
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Rappold I, et al. 1997. Blood 90:111. 2. Rosnet O, et al. 1996. Acta Haematol. 95:218. 3. Rosnet O, et al. 1996. Leukemia 10:238. 4. Bertho JM, et al. 2000. Scand. J. Immunol. 52:53.
Gene ID: 2322
UniProt: View information about CD135 on UniProt.org
Clone: BV10A4H2
Regulatory Status: RUO
Other Names: FMS-like tyrosine kinase-3, FLT-3, STK-1, Flk-2
Isotype: Mouse IgG1, κ