Biolegend, 313520, PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS) Antibody, 100μg

ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.
100μg
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse T cell clone D10.G4.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
Application References(PubMed link indicates BioLegend citation): Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) Yagi J, et al. 2003. J. Immunol. 171:783. (FC) Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed Oh–Hora M, et al. 2019. J Immunol. 202:2616. PubMed Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed Chen J, et al. 2023. Cell Res. 33:341. PubMed Riffelmacher T, et al. 2023. Nat Cell Biol. 25:877. PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed Babl N, et al. 2023. Front Oncol. 13:1107484. PubMed Giles JR, et al. 2022. Immunity. 55:557. PubMed Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed Matsui K, et al. 2015. PLoS One. 10: 0137195. PubMed Myers LM, et al. 2019. Nat Commun. 10:794. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Liu R, et al. 2017. J Neuroimmunol. 10.1016/j.jneuroim.2017.08.005. PubMed Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed Gagne M, et al. 2022. Cell. 185:113. PubMed Sun Z, et al. 2021. eLife. 10:00. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed Herati R, et al. 2014. J Immunol. 193:3528. PubMed Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed Shen X, et al. 2021. Front Immunol. 12:710750. PubMed Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
RRID: AB_10641839 (BioLegend Cat. No. 313519) AB_10643411 (BioLegend Cat. No. 313520)
Structure: CD28/CTLA-4, 47-57 kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95.
Gene ID: 1000488412985164545
UniProt: View information about CD278 on UniProt.org
Clone: C398.4A
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Armenian Hamster IgG