Biolegend, 313568, StarBright UltraViolet 740 anti-human/mouse/rat CD278 (ICOS) Antibody, VO31

This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative . This product is also available for direct purchase within a StarBright Basic Panel , which combines a minimal set of pre-selected compatible reagents. ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.
VO31
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse T cell clone D10.G4.1
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration: 0.6 mg/mL (the concentration relates to the dye component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).
Application References(PubMed link indicates BioLegend citation): Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) Yagi J, et al. 2003. J. Immunol. 171:783. (FC) Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Structure: CD28/CTLA-4, 47-57 kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95.
Gene ID: 1000488412985164545
UniProt: View information about CD278 on UniProt.org
Clone: C398.4A
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Armenian Hamster IgG
Q: What is a “StarBright panel”?
A: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Q: Will my StarBright panel be provided as a cocktail?
A: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Q: Are StarBright GMP/ASR products available?
A: StarBright ASR and GMP products are coming soon.
Q: Are StarBright custom products available?
A: Yes, please contact our Custom Solutions Team.
Q: Will StarBright isotype controls be offered?
A: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
Q: What amount of a StarBright conjugate should I use?
A: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Q: Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?
A: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.