Biolegend, 313711, Alexa Fluor® 647 anti-human CD195 (CCR5) Antibody, 25tests

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Recent studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with human CCR5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1Clone HEK/1/85a and clone HEK/1/85a/7a are the same.
Application References(PubMed link indicates BioLegend citation): Mueller A, et al. 2002. Blood 99:785. Al-Odi E, et al. 2012. PLoS One. 7:e42217. PubMed Osman A, et al. 2014. J Immunol. 192:5083. PubMed Liu S, et al. 2014. Virus Res. 192:92. PubMed
Product Citations: Park A, et al. 2016. Mol Ther Methods Clin Dev. 3:16057. PubMed Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed Piryani SO, et al. 2019. Stem Cell Reports. 13:76. PubMed Mandl M, et al. 2014. PLoS One. 9:112140. PubMed Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed Achour L, et al. 2008. Blood. 101182. PubMed Tadagaki K, et al. 2012. Blood. 119:4908. PubMed Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
RRID: AB_528759 (BioLegend Cat. No. 313711) AB_528760 (BioLegend Cat. No. 313712)
Structure: G-coupled receptor family 1, membrane protein, 45 kD
Distribution: Subset of T cells, monocytes
Function: Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1
Ligand/Receptor: MIP-1α, MIP-1ß, RANTES
Cell Type: Dendritic cells, Monocytes, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661. 5. Lai J, et al. 2003. CVI. 10:1123. 6. Mañes S, et al. 2003. J. Exp. Med. 198:1381. 7. Vaday GG, et al. 2006. Prostate 66:124.
Gene ID: 1234
UniProt: View information about CD195 on UniProt.org
Clone: HEK/1/85a
Regulatory Status: RUO
Other Names: CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype: Rat IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.