Biolegend, 314502, Purified anti-human IgM Antibody, 100μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per 106 in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Application References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations: Mclaurin JD, et al. 2019. Mol Biol Cell. 30:1610. PubMed Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Pearson JA, et al. 2022. Diabetologia. 65:1398. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Pyfrom SC, et al. 2020. J Immunol. 205:595. PubMed Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed Kolhatkar N, et al. 2015. J Exp Med. 212: 1663-1677. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Waugh KA, et al. 2020. Cell Reports. 29(7):1893-1908.e4.. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID: AB_493003 (BioLegend Cat. No. 314502)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507
UniProt: View information about IgM on UniProt.org
Clone: MHM-88
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ