Biolegend, 314505, FITC anti-human IgM Antibody, 25tests

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Application References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations: Sajadi MM, et al. 2018. Cell. 173:1783. PubMed Watanabe A, et al. 2019. Cell. 177:1124. PubMed Nagashima K, et al. 2022. J Immunol. 209:5. PubMed Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed Zhao Y, et al. 2022. Front Immunol. 13:1041176. PubMed Braham MVJ, et al. 2023. iScience. 26:105741. PubMed Sungalee S, et al. 2021. Nat Genet. 53:650. PubMed Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed Muir L, et al. 2018. Wellcome Open Res. 2:97. PubMed Fenwick C, et al. 2022. Nat Microbiol. 7:1376. PubMed Lindner JM, et al. 2019. Immunity. 50:668. PubMed Zost SJ, et al. 2020. Nat Med. 26:1422. PubMed Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed Zost SJ, et al. 2020. bioRxiv. . PubMed Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed Ros F, et al. 2020. MAbs. 12:1846900. PubMed Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed Benhammou JN, et al. 2020. Dig Dis Sci. 66:2394. PubMed Lisboa F, et al. 2011. J Biol Chem. 286:7577. PubMed Nicolò A, et al. 2022. Front Immunol. 13:1016263. PubMed Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed Cizmeci D, et al. 2021. eLife. 10:00. PubMed Fenwick C, et al. 2021. Cell Rep. 37:109814. PubMed Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed Ju L,et al. 2017. Sci Rep.. 10.1038/s41598-017-13793-3. PubMed Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed Song S, et al. 2021. Front Immunol. 12:705140. PubMed
RRID: AB_493009 (BioLegend Cat. No. 314506)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507
UniProt: View information about IgM on UniProt.org
Clone: MHM-88
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ