Biolegend, 314537, Alexa Fluor® 700 anti-human IgM Antibody, 25tests

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
Application References(PubMed link indicates BioLegend citation): Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations: Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed Li L, et al. 2023. PLoS Pathog. e1011856:19. PubMed Wang Z, et al. 2022. J Exp Med. 219: . PubMed Xu C, et al. 2022. iScience. 25:105123. PubMed Schaefer-Babajew D, et al. 2023. Nature. 613:735. PubMed Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed Cho A, et al. 2021. Nature. Online ahead of print. PubMed Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed Wang Z, et al. 2021. Nature. 595:426. PubMed Cho A, et al. 2022. J Exp Med. 219:. PubMed
RRID: AB_2566614 (BioLegend Cat. No. 314537) AB_2566615 (BioLegend Cat. No. 314538)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507
UniProt: View information about IgM on UniProt.org
Clone: MHM-88
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ