Biolegend, 314702, Purified anti-human CD107a (LAMP-1) Recombinant Antibody, 100μg

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Human adult adherent peripheral blood cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA19A32 antibody is able to completely block binding of clone H4A3 on target cells.
RRID: AB_2890749 (BioLegend Cat. No. 314702)
Structure: LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution: Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
Function: Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor: Galaptin
Cell Type: Endothelial cells, Epithelial cells, Lymphocytes, Macrophages, NK cells, Platelets, T cells
Biology Area: Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381. 2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press. 3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675. 4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754. 5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
Gene ID: 3916
UniProt: View information about CD107a on UniProt.org
Clone: QA19A32
Regulatory Status: RUO
Other Names: Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype: Mouse IgG1, κ