Biolegend, 315602, Purified anti-human CD98 Antibody, 100μg

CD98 is a 125 kD disulfide-linked heterodimer protein also known as 4F2 and FRP-1. It is broadly expressed on activated and transformed cells (hematopoietic and non-hematopoietic), and at low levels on quiescent cells. CD98 has been characterized as a potential amino acid transporter (neutral and dibasic amino acids) that is involved in lymphocyte activation and integrin signaling. CD98 is composed of a light chain (45 kD) and a heavy chain (80 kD). Studies with knock-out mice indicate that the CD98 heavy chain is involved in integrin-dependent cell spreading and protection against anchorage deprivation-induced apoptosis. The MEM-108 antibody is useful for flow cytometry and immunoprecipitation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Burkitt's lymphoma cell line Raji
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York. Cantor J, et al. 2011. J. Immunol. 187:851. PubMed.
Product Citations: Weng L, et al. 2018. PLoS Biol. 7:e1428158. PubMed Dhawan K, et al. 2022. Mol Biol Cell. 33:ar107. PubMed Feliciano D, et al. 2021. Nat Commun. 12:4502. PubMed Wunderley L, et al. 2021. J Cell Sci. . PubMed Wunderley L, et al. 2021. J Cell Sci. 134:. PubMed Higashi S, et al. 2022. J Cell Sci. 135:. PubMed Arndt C, et al. 2020. Oncoimmunology. 9:1743036. PubMed Cantor J, et al. 2011. J Immunol. 187:851. PubMed
RRID: AB_2302070 (BioLegend Cat. No. 315602)
Structure: Disulfide-linked heterodimer, 80 kD and 45 kD reduced, 125 kD unreduced
Distribution: Broadly expressed on activated and transformed cells, not hematopoietic cell-specific. Lower levels on resting cells
Function: Potential amino acid transporter (neutral and dibasic amino acids) also involved in the activation of lymphocytes and integrin signaling. May be a target antigen for NK cells
Interaction: Actin, β1 integrins (α2β1, α3β1, α5β1, α6β1; minimally with α4β1)
Modification: Glycosylated (heavy chain)
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997) 2. Bron C, et al. 1986. J. Immunol. 137:397. 3. Lumadue JA, et al. 1987. Proc. Natl. Acad. Sci. 84:9204. 4. Mastroberardino L, et al. 1998. Nature 395:288.
Gene ID: 6520
UniProt: View information about CD98 on UniProt.org
Clone: MEM-108
Regulatory Status: RUO
Other Names: 4F2, FRP-1, RL-388
Isotype: Mouse IgG1, κ