Biolegend, 316210, APC anti-human CD72 Antibody, 100tests

CD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on pre-pre-B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
RRID: AB_2819947 (BioLegend Cat. No. 316209) AB_2819948 (BioLegend Cat. No. 316210)
Structure: Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer
Distribution: Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells
Function: negative regulation of B cell receptor signaling
Ligand/Receptor: CD5, CD100/Sema4D
Cell Type: B cells, Dendritic cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schwarting T, et al. 1992. Am. J. Hematol. 41:151. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155. Kumanogoh A, et al. 2000. Immunity 13:621. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.
Gene ID: 971
UniProt: View information about CD72 on UniProt.org
Clone: 3F3
Regulatory Status: RUO
Workshop: VI CD72.1
Other Names: Lyb-2, Ly-19.2, Ly-32.2
Isotype: Mouse IgG2b, κ