Biolegend, 316510, APC anti-human Ig light chain κ Antibody, 100tests

The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
Product Citations: Oh S, et al. 2023. Nat Biotechnol. :. PubMed Xu Y, et al. 2020. Blood. 136:468. PubMed Roerden M, et al. 2021. Cancers. 13(11):. PubMed Faitschuk E, et al. 2016. Gene Ther. 10.1038/gt.2016.48. PubMed Garfall AL, et al. 2018. JCI Insight. 3. PubMed Bhoj V, et al. 2016. Blood. 128: 360 - 370. PubMed Chakrabarti L, et al. 2022. MAbs. 14:2020081. PubMed
RRID: AB_493614 (BioLegend Cat. No. 316509) AB_493615 (BioLegend Cat. No. 316510)
Structure: Ig family
Distribution: B cells
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3514
UniProt: View information about Ig light chain kappa on UniProt.org
Clone: MHK-49
Regulatory Status: RUO
Other Names: Immunoglobulin light chain kappa
Isotype: Mouse IgG1, κ