Biolegend, 316703, FITC anti-human TCR Vβ5 related subset Antibody, 25tests

The Vβ5 TCR is a variant of the TCR β chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vβ5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR β chain which is expressed on HPB-ALL cells (known expressing TCR Vβ5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR Vβ5.3 antibodies.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human thymoma cell line HPB-ALL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and activation of T cells Vß5 related subset.
Application References(PubMed link indicates BioLegend citation): Pavlistova D, et al. 2003. Immunol. Lett. 88:105.
RRID: AB_528957 (BioLegend Cat. No. 316704)
Structure: Ig superfamily
Distribution: T cell subset
Function: Antigen recognition, activation of TCR Vβ5 bearing T cells
Ligand/Receptor: Some staphylococcus superantigens bound to MHC
Cell Type: T cells, Tregs
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Pavlistova D, et al. 2003. Immunol. Lett. 88:105 2. Kappler T, et al. 1989. Science 244:811 3. Fernandez MM, et al. 2006. Mol. Immunol. 43:927
Gene ID: NA
UniProt: View information about TCR Vbeta5 on UniProt.org
Clone: MEM-262
Regulatory Status: RUO
Other Names: T Cell Receptor Vβ5
Isotype: Mouse IgG2a, κ