Biolegend, 317012, Purified anti-human CD4 Recombinant Antibody, 100μg

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A161A1.RecM1 can completely block the binding of clone A161A1 on target cells.
RRID: AB_3698974 (BioLegend Cat. No. 317012)
Structure: 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution: Helper T cells, majority of thymocytes, monocytes/macrophages, type II NKT cells
Interaction: TCR/CD3 complex, LCK
Ligand/Receptor: MHC class II molecules
Cell Type: Lymphocytes, Macrophages, Monocytes, NKT cells, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area: Adaptive Immunity, Cell Adhesion, Immunology
Molecular Family: CD Molecules
Antigen References: Sattentau QK and Weiss RA. 1988. Cell. 52:631-3. Littman DR, et al. 1987. Annu Rev Immunol. 5:561-84. Zhu J, et al. 2010. Annu Rev Immunol. 28:445-89. Vignali DA. 2010. J Immunol. 184:5933-4.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: A161A1.RecM1
Regulatory Status: RUO
Other Names: T4/Leu-3
Isotype: Mouse IgG1, κ