Biolegend, 317113, Brilliant Violet 421™ anti-human CD158/h (KIR2DL1/KIR2DS1) Antibody, 25tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans. CD158a and CD158h are 38-50 kD two extracellular Ig domain single-pass glycoprotein that contains a short cytoplasmic tail for KIR2DL1. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL1 and KIR2DS1 have been reported to bind certain HLA-C antigens on target cells (HLA-C*02, HLA-C*04, HLAC*06, for example).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human KIR2DS1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S22013A can block the binding of clone HP-DM1 on target cells and is not blocked by HP-DM1. Based on in-house testing on different KIR-expressing transfectants, clone S22013A does not interact with other KIR protein family members.
RRID: AB_3720564 (BioLegend Cat. No. 317113) AB_3720564 (BioLegend Cat. No. 317114)
Structure: 38-50 kD two Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, subset of T cells
Function: NK cell regulation
Ligand/Receptor: Some HLA-C molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Vilches C, et al. 2002. Annu. Rev. Immunol. 92.107361. Middleton D, et al. 2010. Immunol. 123:8. Meenagh A, et al. 2008. Tissue Antigens. 72.4. Le Luduec J.B, et al. 2019. Frontiers Immu. 10.734. Chewning J.H, et al. 2007. Journals of Immu. 179.2.
Gene ID: 38023806
UniProt: View information about CD158a CD158h on UniProt.org
Clone: S22013A
Regulatory Status: RUO
Other Names: CD158a (KIR2DL1, NKAT1, p58.1, KIR221, KIR-K64), CD158h (KIR2DS1, p50.1)
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.