Biolegend, 317314, Pacific Blue™ anti-human CD3 Antibody, 100μg

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Li B, et al. 2005. Immunology 116:487. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed Alter G, et al. 2008. J. Virol. 82:9668. PubMed Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed Pinto JP, et al. 2010. Immunology. 130:217. PubMed Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Beck C, et al. 2023. Biomedicines. 11:. PubMed Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed Hope C, et al. 2014. Kidney Int. 86:175. PubMed Xia Q, et al. 2022. Methods Mol Biol. 2574:251. PubMed Collins D, et al. 2015. PLoS Pathog. 11: 1005054. PubMed Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed Bernardes JP, et al. 2020. Immunity. 53:1296. PubMed LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed Zaikos TD, et al. 2018. Cell Rep. 25:3759. PubMed Daniels J, et al. 2020. Nat Commun. 11:1806. PubMed Welbourn S, et al. 2022. PLoS Pathog. 18:e1010488. PubMed
RRID: AB_571908 (BioLegend Cat. No. 317313) AB_571909 (BioLegend Cat. No. 317314)
Structure: Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: OKT3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG2a, κ