Biolegend, 317319, Biotin anti-human CD3 Antibody, 25μg

CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Li B, et al. 2005. Immunology 116:487. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed Alter G, et al. 2008. J. Virol. 82:9668. PubMed Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed Pinto JP, et al. 2010. Immunology. 130:217. PubMed Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations: Zhang DKY, et al. 2020. Nature Protocols. 15(3):773-798.. PubMed Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Banta KL, et al. 2022. Immunity. 55:512. PubMed Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed McAffee DB, et al. 2022. Nat Commun. 13:7446. PubMed Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed Dong R, et al. 2020. EMBO J. 39:e104730. PubMed Xu X, et al. 2021. Elife. 10:. PubMed Motsch V, et al. 2019. Sci Rep. 2.658333333. PubMed Serwas NK, et al. 2019. Nat Commun. 2.573611111. PubMed Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed Allegrezza M, et al. 2016. Cancer Res . 76: 2561 - 2572. PubMed Zheng Y, et al. 2021. Nat Commun. 12:3580. PubMed Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID: AB_10918432 (BioLegend Cat. No. 317319) AB_10916519 (BioLegend Cat. No. 317320)
Structure: Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: OKT3
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.