Biolegend, 317413, PE/Cyanine7 anti-human CD4 Antibody, 25tests

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
25tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human peripheral T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations: Spitsin S, et al. 2020. Mol Ther Methods Clin Dev. 17:1088. PubMed Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed Li M, et al. 2023. Front Immunol. 14:1087923. PubMed Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed Ataya M, et al. 2020. Am J Transplant. 20:663. PubMed Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed Kwee BJ, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed Swadling L, et al. 2020. Cell Rep. 30:687. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Lieberman L, et al. 2016. Neurology. 86: 375 - 381. PubMed Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed Soriano-Sarabia N, et al. 2012. J Immunol. 189:5212. PubMed Konadu K, et al. 2015. J Infect Dis. 211:1712. PubMed Della–Torre E, et al. 2018. Arthritis Rheumatol. 70:1133. PubMed Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed Bellini N, et al. 2022. iScience. 25:105234. PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Ellis-Connell AL, et al. 2018. J Virol. 92:e01748. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Morimoto Y, et al. 2018. Immunity. 49:134. PubMed Blom L, et al. 2011. PLoS One. 6:e21695. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Hirata SI, et al. 2020. Allergy. 75:1939. PubMed Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed Podaza E, et al. 2020. Front Immunol. 11:1147. PubMed Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed Coiras M, et al. 2016. Cell Rep. 14:2100-2107. PubMed Keskin DB, et al. 2019. Nature. 565:234. PubMed Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
RRID: AB_571958 (BioLegend Cat. No. 317413) AB_571959 (BioLegend Cat. No. 317414)
Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: OKT4
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T4
Isotype: Mouse IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.