Biolegend, 317418, APC/Cyanine7 anti-human CD4 Antibody, 100tests

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human peripheral T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations: Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed He WT, et al. 2022. Sci Transl Med. 14:eabl9605. PubMed Zhou P, et al. 2023. Immunity. 56:669. PubMed Zhang M, et al. 2023. JCI Insight. 8: . PubMed Wang P, et al. 2023. Front Immunol. 13:1104329. PubMed Gilman KE, et al. 2023. Front Immunol. 13:1045710. PubMed Zhou X, et al. 2021. Cell Reports. 35(6):109109. PubMed Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed García-Broncano P, et al. 2020. J Infect. 80:99. PubMed Hoang TN, et al. 2021. Cell. 184:460. PubMed Sampedro-Nuñez M, et al. 2018. Sci Rep. 8:17812. PubMed Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed Wang H, et al. 2022. Front Immunol. 13:852436. PubMed Pino M, et al. 2022. Nat Commun. 13:5055. PubMed Yuan X, et al. 2022. J Extracell Vesicles. 11:e12235. PubMed Pino M, et al. 2020. Front Immunol. 11:1275. PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Boyle M, et al. 2015. PLoS Pathog. 11: 1005041. PubMed Cicin-Sain L, et al. 2010. J Immunol. 184:6739. PubMed Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed Song G, et al. 2020. bioRxiv. . PubMed Hoang TN, et al. 2020. bioRxiv. . PubMed Richert-Spuhler LE, et al. 2021. Cell Reports Medicine. 2(6):100322. PubMed Bäcker-Koduah P, et al. 2020. Ann Clin Transl Neurol. 1.422222222. PubMed Shen X, et al. 2021. Front Immunol. 12:710750. PubMed Leclercq G, et al. 2021. J Immunother Cancer. 9: . PubMed Harper JL, et al. 2020. Nat Med. 519:26. PubMed Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed He WT, et al. 2022. Nat Immunol. 23:960. PubMed Moore T,et al. 2017. Cancer Immunol Immunother. . 10.1007/s00262-017-2073-0. PubMed Franco LM, et al. 2019. J Exp Med. 216:384. PubMed Ostadkarampour M, et al. 2016. PLoS One. 11:e0164751. PubMed Xue J, et al. 2013. PLoS One. 8:72295. PubMed Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed Chen YP, et al. 2020. Cell Res. 30:1024. PubMed Su C, et al. 2020. Nat Commun. 2.745833333. PubMed Zhao F, et al. 2020. Cell Rep. 32:108122. PubMed Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed Wu B, et al. 2022. Nat Commun. 13:2155. PubMed Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed Chowdhury A, et al. 2015. J Virol. 89: 8677-8686. PubMed Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed Yost KE, et al. 2019. Nat Med. 25:1251. PubMed
RRID: AB_571946 (BioLegend Cat. No. 317417) AB_2687202 (BioLegend Cat. No. 317450) AB_571947 (BioLegend Cat. No. 317418)
Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: OKT4
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T4
Isotype: Mouse IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.