Biolegend, 317902, Purified anti-BrdU Antibody, 100μg

BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. The MoBU-1 antibody is useful for the detection of incorporated BrdU by fluorescent cell staining (ICFC), flow cytometry, and immunohistochemistry.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC, ICFC - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Swee LK, et al. 2009. Blood 113:6277. PubMed Basile V, et al. 2009. Biochem Pharmacol. 78:1035. PubMed 3. Benatti P, et al. 2011. Nucelic Acid Res. PubMed Tahirovic S, et al. 2010. J. Neurosci. 30:6930. (IHC) Harms G and Hardonk MJ. 1989. J. Histochem. Cytochem. 37:479. (IHC) Wang H, et al. 2014. PLoS One. 9:109697. PubMed
Product Citations: Pibuel MA, et al. 2023. Sci Rep. 13:9356. PubMed Nam HS, et al. 2023. Neural Dev. 18:1. PubMed Wang H, et al. 2014. PLoS One. 9:109697. PubMed Basile V, et al. 2016. Biochim Biophys Acta. 1859: 627-638. PubMed Gheller BJ, et al. 2021. Mol Metab. 43:101106. PubMed Oliveira KMC, et al. 2019. Sci Rep. 9:11433. PubMed AR F, et al. 2016. Sci Rep. 6: 32900. PubMed Hodel KP, et al. 2020. Molecular Cell. 78(6):1166-1177.e6. PubMed Zhou T, et al. 2020. eLife. 9:e52779.. PubMed Pibuel MA, et al. 2021. Cell Death Discov. 7:280. PubMed Benatti P, et al. 2011. Nucleic Acids Res. 39:5356. PubMed Basile V, et al. 2009. Biochem Pharmacol. 78:1305. PubMed Ivanova A, et al. 2022. Cells. 11:. PubMed Swee L, et al. 2009. Blood. 113:6227. PubMed
RRID: AB_604040 (BioLegend Cat. No. 317902)
Structure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Function: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area: Cell Biology, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.
Gene ID: NA
Clone: MoBU-1
Regulatory Status: RUO
Other Names: Bromodeoxyuridine
Isotype: Mouse IgG1, κ