Biolegend, 318103, FITC anti-human HLA-DQ Antibody, 25tests

HLA-DQ is also known as MHC class II DQ monomorphic antigen. The major histocompatibility complex is composed of two heterodimeric glycoproteins (α and β chains) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic, with the α chain showing an extremely high degree of polymorphism. HLA-DQ is expressed on B cells in the peripheral blood, and weakly expressed on activated T cells and some monocytes. HLA-DQ is absent on hematopoietic progenitors, resting T cells, erythrocytes, and platelets. HLA-DQ is expressed after HLA-DR and HLA-DP in hematopoietic development. HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ T lymphocytes. Specific alleles of HLA-DQ have been linked to the pathogenesis of several autoimmune diseases (including diabetes), both as a susceptibility and resistance factor depending on the particular polymorphism.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The antibody HLADQ1 reacts with HLA-DQ 4,5,6,7,8,9 but does not react with DQ2, DR or DP. Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissues.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leukocyte Typing IV. Oxford University Press. New York.
Product Citations: Li J, et al. 2021. Front Cell Dev Biol. 9:737242. PubMed Li J, et al. 2022. Int J Mol Sci. 23:. PubMed Porter K, et al. 2010. J Immunol. 185:6480. PubMed Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed Kim M, et al. 2012. J Immunol. 188:4158. PubMed
RRID: AB_604128 (BioLegend Cat. No. 318104)
Structure: Major histocompatability complex composed of two heterodimeric glycoproteins (α and β chain) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic.
Distribution: Expressed on B cells in the peripheral blood, weakly on activated T cells and some monocytes. Absent on hematopoietic progenitors, resting T cells, erythrocytes and platelets. Expressed after HLA-DR and HLA-DP in hematopoietic development.
Function: HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ lymphocytes. Specific alleles are linked to the pathogenesis of several autoimmune diseases.
Interaction: CD3/TCR, CD4
Cell Type: Thymocytes, T cells, Leukemia
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. So AK, et al. 1987. J. Immunol. 139:3506. 2. Gyllensten UB Erlih HA. 1989. Proc. Natl. Acad. Sci. USA 86:9986. 3. Sonderstrup G and McDevitt HO. 2001. J. Clin. Invest. 107:795.
Gene ID: 31173119
UniProt: View information about HLA-DQ on UniProt.org
Clone: HLADQ1
Regulatory Status: RUO
Other Names: MHC class II DQ monomorphic antigen, MHC II
Isotype: Mouse IgG1, κ