Biolegend, 318209, Purified anti-human CD171 (L1CAM) Antibody, 100μg

CD171, also known as L1CAM or L1, is a 200-220 kD neuronal cell adhesion molecule and member of the L1 protein family. This transmembrane glycoprotein plays an important role in nervous system development, including neuron-neuron adhesion, signal transduction, axon guidance, cell migration and differentiation. Mutations in the CD171 gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. CD171 is also strongly implicated in treatment-resistant cancers.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human L1CAM/CD171, amino acid Ile20-Glu1120 (Accession No. NM_024003.2) and a C-terminal Fc tag was expressed in 293E cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3698978 (BioLegend Cat. No. 318209)
Structure: 200-220 kD. Several immunoglobulin-like domains and fibronectin-like repeats (type III), linked via a single transmembrane sequence to a conserved cytoplasmic domain.
Distribution: Tetanus-toxin positive neurons, endothelial cells, certain epithelial cells, reticular fibroblasts, colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin
Function: Adhesion molecule. Homotypic and heterotypic cell-cell interaction in neuronal myelination, neurite outgrowth and regeneration. Axonal guidance, neuronal migration.
Ligand/Receptor: Homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules.
Cell Type: Endothelial cells, Epithelial cells, Fibroblasts, Neurons
Biology Area: Cancer Biomarkers, Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Wolterink S, et al. 2010. Cancer Res. 70:2504-15. Huszar M, et al. 2006. Human Pathology. 37:1000. Kaifi JT, et al. 2006. Anticancer Res. 26:1167-70. Kaifi JT, et al. 2006. Mod. Pathol. 3:399-406. Meier F, et al. 2006. Int J Cancer. 119:549-55. Stoeck A, et al. 2006. Biochem J. 393:609-18. Gutwein P, et al. 2005. Clin. Cancer Res. 11:2492-501.
Gene ID: 3897
UniProt: View information about CD171 on UniProt.org
Clone: A21047G
Regulatory Status: RUO
Other Names: CAML1, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, S10, SPG1, L1CAM, Neural cell adhesion molecule L1
Isotype: Mouse IgG1, κ