Biolegend, 318332, APC/Cyanine7 anti-human CD56 (NCAM) Antibody, 100tests

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations: Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed Bayik D, et al. 2022. Cancer Res. 82:4274. PubMed Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed Hastie KM, et al. 2023. Cell Rep. 42:112421. PubMed McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed Boisson B, et al. 2015. J Exp Med. 212:939. PubMed Kreutmair S, et al. 2021. Immunity. . PubMed Barton J, et al. 2019. F1000Res. 8:823. PubMed Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed Port JR, et al. 2020. J Virol. 94:. PubMed Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed Alonso R, et al. 2022. Blood Adv. :. PubMed Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed Edgar C, et al. 2015. PLoS One. 10:117614. PubMed Li Y, et al. 2022. J Immunol. 208:347. PubMed Manuszak C, et al. 2020. J Biol Methods. 7:e131. PubMed Hataye JM, et al. 2020. Cell Host & Microbe. 26(6):748-763.e20.. PubMed Corrado M, et al. 2020. Cell Metab. 32:981. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID: AB_10898118 (BioLegend Cat. No. 318331) AB_10896424 (BioLegend Cat. No. 318332)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparin sulfate
Cell Type: B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: HCD56
Regulatory Status: RUO
Other Names: Leu-19, NKH1
Isotype: Mouse IgG1, κ